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Public release date: 1-Nov-2011[ | E-mail |
Share ] Contact: Lindsay Key
ltkey@vt.edu
540-231-6594
Virginia Tech
The next time you take aspirin for a headache, thank a willow tree. Salicylic acid, a compound chemically similar to aspirin, is found in willow tree bark and is made by the plant as a chemical defense against pathogens. By mimicking the chemical production processes of plants, scientists have been able to synthetically produce and engineer many important alkaloid drug products, including caffeine, atropine (an anti-spasmodic used to treat heart arrhythmia), nicotine, morphine and quinine.
The trick, however, is to understand how a plant produces these compounds and then transports them through the plant. A team of Virginia Tech and Purdue University scientists have identified a distinct transporter used by tobacco plant cells for nicotine metabolism. The research appears in the Oct. 17 online before print issue of the Proceedings of the National Academy of Sciences (PNAS) in the article, "A tobacco nicotine uptake permease effects alkaloid metabolism."
The transporter, called nicotine uptake permease (NUP1) allows alkaloids nitrogen-containing compounds to pass into plant cells. The transporter is a tubular structure found in the outer boundary of the plant cell, known as the plasma membrane. Passage of alkaloids from one cell to another in an assembly line fashion is crucial to the biosynthesis of many medicinal plant alkaloids. However, prior to the study, it was not known exactly how the alkaloids were able to move either in or out of the cell.
"We knew that the general process of going in and out is important for alkaloid metabolism, but we didn't know the details," said John Jelesko, associate professor of plant pathology, physiology, and weed science at Virginia Tech. "The NUP1 transporter is very specific for nicotine other closely related alkaloids don't use this transporter. That degree of specificity is rather unusual."
Identification of the transporter is a first step in understanding details about how the tobacco plant manages nicotine transport processes. So far, it is known that the chemical is made in the roots of tobacco plants, as a response to a pathogen attack. When ingested by insects and other organisms, it is a powerful neurotoxin, causing paralysis. Once produced in the roots, it is transported up through the rest of the plant by tube-like passageways called xylem and accumulates in the leaves. Tobacco farmers harvest nicotine by collecting the plant's leaves.
The scientists were able to locate the transporter using a "guilt by association" tactic, Jelesko said. The NUP1 gene was identified by looking for genes that were turned up or down in concert with known nicotine biosynthesis genes. The discovery of the transporter helps scientists gain new insight into the overarching field of medicinal alkaloid production in plants, Jelesko said. Understanding the complete process could enable bioengineering of medicinal plants to produce an optimal amount of compounds beneficial for treatment.
###
Research team leaders with Jelesko were Sakiko Okumoto, assistant professor of plant pathology, physiology, and weed science at Virginia Tech, and Angus Murphy, professor of horticulture at Purdue University.
Co-authors in order are Sherry B. Hildreth, a postdoctoral associate in biological sciences at Virginia Tech who received her Ph.D. in plant pathology, physiology, and weed science at Tech in 2009; Elizabeth A. Gehman, a former technician in plant pathology, physiology, and weed science at Virginia Tech, now with the Battelle National Biodefense Institute, National Biodefense Analysis and Countermeasures Center; Haibing Yang, a research scientist with the horticulture department at Purdue; Rong-He Lu, former technician in plant pathology, physiology, and weed science at Virginia Tech; Ritesh KC, research specialist in plant pathology, physiology, and weed science at Virginia Tech; Kim Harich, analytical chemist in the biochemistry department at Virginia Tech; Shi Yu, Ph.D. student in plant physiology at Virginia Tech; Jinshan Lin, Ph.D. student in horticulture at Purdue; Jackson L. Sandoe, a former undergraduate student in biological sciences at Virginia Tech, now a graduate student in molecular and cellular biology at Harvard University; Okumoto; Murphy; and Jelesko, the corresponding author.
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Public release date: 1-Nov-2011[ | E-mail |
Share ] Contact: Lindsay Key
ltkey@vt.edu
540-231-6594
Virginia Tech
The next time you take aspirin for a headache, thank a willow tree. Salicylic acid, a compound chemically similar to aspirin, is found in willow tree bark and is made by the plant as a chemical defense against pathogens. By mimicking the chemical production processes of plants, scientists have been able to synthetically produce and engineer many important alkaloid drug products, including caffeine, atropine (an anti-spasmodic used to treat heart arrhythmia), nicotine, morphine and quinine.
The trick, however, is to understand how a plant produces these compounds and then transports them through the plant. A team of Virginia Tech and Purdue University scientists have identified a distinct transporter used by tobacco plant cells for nicotine metabolism. The research appears in the Oct. 17 online before print issue of the Proceedings of the National Academy of Sciences (PNAS) in the article, "A tobacco nicotine uptake permease effects alkaloid metabolism."
The transporter, called nicotine uptake permease (NUP1) allows alkaloids nitrogen-containing compounds to pass into plant cells. The transporter is a tubular structure found in the outer boundary of the plant cell, known as the plasma membrane. Passage of alkaloids from one cell to another in an assembly line fashion is crucial to the biosynthesis of many medicinal plant alkaloids. However, prior to the study, it was not known exactly how the alkaloids were able to move either in or out of the cell.
"We knew that the general process of going in and out is important for alkaloid metabolism, but we didn't know the details," said John Jelesko, associate professor of plant pathology, physiology, and weed science at Virginia Tech. "The NUP1 transporter is very specific for nicotine other closely related alkaloids don't use this transporter. That degree of specificity is rather unusual."
Identification of the transporter is a first step in understanding details about how the tobacco plant manages nicotine transport processes. So far, it is known that the chemical is made in the roots of tobacco plants, as a response to a pathogen attack. When ingested by insects and other organisms, it is a powerful neurotoxin, causing paralysis. Once produced in the roots, it is transported up through the rest of the plant by tube-like passageways called xylem and accumulates in the leaves. Tobacco farmers harvest nicotine by collecting the plant's leaves.
The scientists were able to locate the transporter using a "guilt by association" tactic, Jelesko said. The NUP1 gene was identified by looking for genes that were turned up or down in concert with known nicotine biosynthesis genes. The discovery of the transporter helps scientists gain new insight into the overarching field of medicinal alkaloid production in plants, Jelesko said. Understanding the complete process could enable bioengineering of medicinal plants to produce an optimal amount of compounds beneficial for treatment.
###
Research team leaders with Jelesko were Sakiko Okumoto, assistant professor of plant pathology, physiology, and weed science at Virginia Tech, and Angus Murphy, professor of horticulture at Purdue University.
Co-authors in order are Sherry B. Hildreth, a postdoctoral associate in biological sciences at Virginia Tech who received her Ph.D. in plant pathology, physiology, and weed science at Tech in 2009; Elizabeth A. Gehman, a former technician in plant pathology, physiology, and weed science at Virginia Tech, now with the Battelle National Biodefense Institute, National Biodefense Analysis and Countermeasures Center; Haibing Yang, a research scientist with the horticulture department at Purdue; Rong-He Lu, former technician in plant pathology, physiology, and weed science at Virginia Tech; Ritesh KC, research specialist in plant pathology, physiology, and weed science at Virginia Tech; Kim Harich, analytical chemist in the biochemistry department at Virginia Tech; Shi Yu, Ph.D. student in plant physiology at Virginia Tech; Jinshan Lin, Ph.D. student in horticulture at Purdue; Jackson L. Sandoe, a former undergraduate student in biological sciences at Virginia Tech, now a graduate student in molecular and cellular biology at Harvard University; Okumoto; Murphy; and Jelesko, the corresponding author.
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Source: http://www.eurekalert.org/pub_releases/2011-11/vt-prl110111.php
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